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3 edition of A model for isolated rat liver perfusion in normo- and hypothermia found in the catalog.

A model for isolated rat liver perfusion in normo- and hypothermia

Gerardus Nicolaas de Ruijter

A model for isolated rat liver perfusion in normo- and hypothermia

description and application in liver preservation research

by Gerardus Nicolaas de Ruijter

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  • 32 Currently reading

Published by Rijksuniversiteit te Groningen in Groningen .
Written in English


Edition Notes

Thesis (Ph.D.) - Rijksuniversiteit te Groningen, 1989.

Statementdoor Gerardus Nicolaas de Ruijter.
ID Numbers
Open LibraryOL20189178M
ISBN 109090029877
OCLC/WorldCa46112261

In contrast, mild hypothermia caused a continuous PmyO2 decrease from initially ± mmHg to ± mmHg (−%, p normo- and hypothermic ECC. The effects of acetylcholine on glucose and lactate balance and on perfusion flow were studied in isolated rat livers perfused simultaneously via the hepatic artery ( mmHg, % of flow) and the portal vein (10 mmHg, % of flow) with a Krebs-Henseleit bicarbonate buffer containing 5 mM-glucose, 2 mM-lactate and mM-pyruvate.

Although mild hypothermia reduces inflammation –, detrimental effects of deep hypothermia have been reported in experimental settings, and in humans. In accordance, induction of deep hypothermia that paralleled effects of NaHS either worsened lung inflammation or had no effect in a rat model of ventilator induced lung injury [18], [43] or. The Year Book of Critical Care Medicine 31 Sjöberg T, Norgren L, Andersson K-E, Steen S: Comparative effects of the a-adrenoceptor agonists noradrenaline, phenylephrine and clonidine in the human saphenous vein in vivo and in vitro.

PERFUSION hypothermia is the means by which body temperature can be lowered by selectively cooling the temperature of blood passing through an extracorporeal circuit. It has been long recog-nized that mild perfusion hypothermia (decreasing temperature to around 30°C) produces a decrease in extracorporeal reservoir blood volume at con-. 2. Alpini G, Garrick RA, Jones MJ, et al. Water and nonelectrolyte permeability of isolated rat hepatocytes. Amer J Physiol ; 4. Bailes JE, Leavitt ML, Teeple E Jr, et al. Ultraprofound hypothermia with complete blood substitution in a canine model. J Neurosurg ; 5.


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A model for isolated rat liver perfusion in normo- and hypothermia by Gerardus Nicolaas de Ruijter Download PDF EPUB FB2

By combining these bed-side parameters we were able to determine the functional capacity of an isolated liver properly. Under moderate hypothermia (34 degree to 35 degree C) the oxygen consumption falls by 25%, while the bile secretion falls by %.

The success of an extracorporeal liver perfusion will depend on achieving normothermal Author: T Wustrow, M Fischer, W Erhardt, G Blümel. This thesis concerns the development and application of a research model for normo- (37°C) and hypothermic (36°C and below) perfusion of the isolated rat liver.

three questions were put. Firstly, what is the effect of cooling on function and morphology of the isolated perfused rat liver.

Author: Gerardus Nicolaas de Ruijter. A model for isolated rat liver perfusion in normo- and (). Pagina-navigatie: Main; Save publication.

Save as MODS; Export to Mendeley; Save as EndNote; Export to RefWorks; Title: A model for isolated rat liver perfusion in normo- and hypothermia.

Description and application in liver preservation research: Author: Ruijter, Gerardus Author: Gerardus Nicolaas de Ruijter. A model for isolated rat liver perfusion in normo- and hypothermia. Description and application in liver preservation researchAuthor: Gerardus Nicolaas de Ruijter.

system of isolated perfused rat liver; mitochondria were extracted from the livers and studied by using top-down control analysis.

During the temperature transition from hypothermic to normo. Hypothermia may attenuate the progression of ischemia-induced damage in liver. Here, we determined the effects of a brief cycle of hypothermic preconditioning applied before an ischemic/reperfusion (I/R) episode in isolated perfused rat liver (IPRL) on tissue damage and oxidative stress.

Rats (male, – g) were anaesthetised with sodium pentobarbital (60 mgkg−1 i.p) and underwent. PDF | BACKGROUND Machine perfusion techniques offer a solution to the serious organ shortage.

However, to assess the effects of machine perfusion, many | Find, read and cite all the research. Purpose. To develop a viable, single pass rat head perfusion modeluseful for pharmacokinetic studies.

Methods. A viable rat head preparation, perfused with MOPS-bufferedRinger's solution, was developed. Radiolabelled markers (red bloodcells, water and sucrose) were injected in a bolus into the internalcarotid artery and collected from the posterior facial vein over 28minutes.

Hepatocytes from isolated rat livers were hypothermically incubated (5 °C) in an oxygenated environment with continuous shaking (to simulate organ perfusion preservation).

The incubation solution was either a tissue culture medium (L), an organ preservation perfusate (UW gluconate), or a simple cold-storage solution used for organ.

BACKGROUND: Previous studies revealed evidence that induced hypothermia attenuates ischemic organ injuries after severe trauma. In the present study, the effect of hypothermia on liver damage was investigated in a porcine long term model of multi-system injury, consisting of blunt chest trauma, penetrating abdominal trauma, musculoskeletal injury, and hemorrhagic shock.

Hepatocytes isolated from the rat liver were stored for up to 72 hr at 4 °C in a tissue culture medium (Liebovitz) at different pH values to determine how pH affects hepatocyte viability.

This is a model to simulate cold storage of livers for transplantation and determine the optimal pH for maintenance of liver cell function. tures during 6 h of oxygenated machine perfusion in a rat liver model [27]. Lifor at 21 °C improved cellular structure, increased bile production and lowered enzyme release; however, there was an increase in levels of lactate after per-fusion which questions its efficiency as an oxygen carrier.

AQIX-RS-I is nonphosphate-buffered solution. For these experiments we used the perfusion model described by Kessler (2) and Lang (3). After a short initial ischemia associated with preparation of the organ we perfused the liver with an O 2-saturated medium for 30 min, then for one hour with a N 2-saturated medium.

Conclusion – A simplified model of liver isch-emia/reperfusion that simulates orthotopic liver autotransplantion was demonstrated.

Topical hypothermia of isolated hepatic lobules showed liver protection, being a viable and practical method for any kind of in vivo liver preservation study. HEADINGS – Ischemia. Reperfusion injury.

Zapletal et al. studied the effect of selenium on liver function in the model of partial warm liver ischemia in the rat. Sodium selenite was administered to rats intravenously at a dose of µg/g, µg/g and µg/g.

An improvement in hepatic microcirculation was observed. Hypothermia may protect the microvasculature by reducing postischemic endothelial injury due to oxidative stress. Zar et al. have examined the effects of mild hypothermia in an isolated rat liver model of ischemia and reperfusion. Data from their studies show that hypothermic perfusion decreased the formation of reactive oxygen species and.

In a cold ex-vivo rat liver perfusion model and a syngeneic liver transplant OLT model, treatment of genetically obese Zucker rats with Ad-HO-1 improved portal venous blood flow, increased bile production, and decreased hepatocyte injury.

Unlike in untreated rats, upregulation of HO-1 correlated with preserved hepatic architecture, improved. Using perfusion microreactors that are seeded with primary rat hepatocytes, these cells can be maintained demonstrating greater functional activity than cells in conventional 2-D culture.

When liver-derived endothelial cells are added, they proliferate and form microvessel-like networks within 3-D reactor cultures, maintaining. Pig hearts from the abattoir: another approach to an isolated perfusion model Robert Rauh, Michael Hiller, Tobias Trinks, Manfred Kessler Proc.

SPIE.Functional Monitoring and. In the 2-VO model, both the ischemia and reperfusion are almost immediate, and the model has been utilized as an alternate to the 4-VO model of forebrain ischemia (Smith et al. Cerebral blood flow, when measured between 5 and 15 min of ischemia, is decreased to less than 5% of control in cortex, and to a lesser degree in thalamus and.

d human donor livers preserved in University of Wisconsin solution(UW) was assessed shortly before implantation using noninvasive 31P magnetic resonance spectroscopy.

We conclude that tissue pH during cold storage may be partly dependent upon hepatic glycogen stores and donor age.

The wide range of tissue pH values that was observed at the time of implantation does not result in significant.Abstract: For many years, the isolated perfused rat liver (IPRL) model has been used to investigate the physiology and pathophysiology of the rat liver.

This in vitro model provides the opportunity to assess cellular injury and liver function in an isolated setting. Cell Mol. Biol. – to the model design. Meningitis was induced 16 hours 4.

Jaeschke, H., A. Farhood, and C. W. Smith. Neutrophils con- prior to condition randomization. Animals randomized tribute to ischemia-reperfusion injury in rat liver in vivo. FASEB to hypothemic conditions demonstrated down-regulation J.

– 5.